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apc  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec apc
    Apc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1550 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd133+apc/CD133%2F1+Antibody%2C+anti-human/pmc13406482-20-6-10
    Average 95 stars, based on 1550 article reviews
    apc - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Flow Cytometry:

    Article Title: Treatment of glioblastoma with tumor-specific amplitude-modulated radiofrequency electromagnetic fields
    Article Snippet: .. After seven days of treatment cells were labelled for NESTIN-AF488 (mouse anti-human 1:50 (U251 and BTCOE-4795), Cat#53-9843 Affymetrix eBioscience) and CD133-APC (mouse anti-human 1:100 (U251 and BTCOE-4795) Cat# 130–098-826 Miltenyi Biotec) markers of cancer stem cell, fixed and analyzed via flow cytometry. .. Data collection was performed on a C6 accrui flow cytometer while analysis was performed on CFlow Plus software (Becton Dickinson).

    Article Title: Anti-cancer effect of Mesenchymal Stem
    Article Snippet: Assessing differentiation potential of MSCs The cultured cells were differentiated into osteogenic, adipogenic and chondrogenic lineage by culturing in osteogenic medium [DMEM supplemented with 10− 8 M dexamethasone (Sigma, D4902), 10 mM β glycerophosphate (Sigma, G9422), and 50 μg/ml ascorbic acid], adipogenic medium [DMEM supplemented with 10 mM 3 isobutyl-1-methylxanthine (Sigma, 17018), 0.1 mM indomethacin (Sigma, 17378), 10 μg/ml insulin (Sigma, I6634), 10− 6 dexamethasone] and chondrogenic medium (Stempro, Invitrogen) and con rmed by staining with Alizarin red (Sigma, A5533), Oil red O (Sigma, O0625) and Alcian blue (Himedia Laboratories, Mumbai, India: Cat no RM471-1 g staining, respectively). .. Flow cytometry analysis For surface marker immunophenotyping, cells were stained with the following conjugated antibodies: anti-CD45-vioblue, anti-CD34-PE, HLADr-vioblue, anti-CD73-PE, anti-CD90-FITC, anti-CD105-vioblue, CD24PE CD44-FITC, CD133-APC, CD14-PE and relevant isotypes (Miltenyi-Biotec). ..

    Article Title: KRAS-driven pancreatic ductal adenocarcinoma cell lines harbour putative cancer stem cells
    Article Snippet: .. Single cells (1 × 106/100 μL) were stainedwith human CD44FITC (Cat# 130-113-334, RRID: AB_2726110, Miltenyi Biotec), CD24-PE (Cat# 130-095-953, RRID: AB_10828818, Miltenyi Biotec), and CD133-APC (Cat# 130-113-106, RRID: AB_2725935, Miltenyi Biotec) antibodies (Miltenyi Biotec, Germany) by diluting the antibodies at 1:50 ratio in fluorescence-activated cell sorting (FACS) staining buffer: 0.5 % (w/v) BSA (Sigma-Aldrich, USA), 2 mM EDTA (SigmaAldrich, USA) in 1 × phosphate-buffered saline (PBS; pH 7.4) at 4 °C in the dark for 10 min. Dead cells were stained with 5 μL (0.25 μg) 7-Aminoactinomycin D (7-AAD; BD Biosciences, USA) at 4 °C in the dark for 10 min. Data were acquired by using the BD FACSCantoTM II flow cytometer (BD Biosciences, USA) and analysed with BD FACSDivaTM software (version 8.0, RRID: SCR_001456, BD Biosciences, USA). ..

    Marker:

    Article Title: Anti-cancer effect of Mesenchymal Stem
    Article Snippet: Assessing differentiation potential of MSCs The cultured cells were differentiated into osteogenic, adipogenic and chondrogenic lineage by culturing in osteogenic medium [DMEM supplemented with 10− 8 M dexamethasone (Sigma, D4902), 10 mM β glycerophosphate (Sigma, G9422), and 50 μg/ml ascorbic acid], adipogenic medium [DMEM supplemented with 10 mM 3 isobutyl-1-methylxanthine (Sigma, 17018), 0.1 mM indomethacin (Sigma, 17378), 10 μg/ml insulin (Sigma, I6634), 10− 6 dexamethasone] and chondrogenic medium (Stempro, Invitrogen) and con rmed by staining with Alizarin red (Sigma, A5533), Oil red O (Sigma, O0625) and Alcian blue (Himedia Laboratories, Mumbai, India: Cat no RM471-1 g staining, respectively). .. Flow cytometry analysis For surface marker immunophenotyping, cells were stained with the following conjugated antibodies: anti-CD45-vioblue, anti-CD34-PE, HLADr-vioblue, anti-CD73-PE, anti-CD90-FITC, anti-CD105-vioblue, CD24PE CD44-FITC, CD133-APC, CD14-PE and relevant isotypes (Miltenyi-Biotec). ..

    Staining:

    Article Title: Anti-cancer effect of Mesenchymal Stem
    Article Snippet: Assessing differentiation potential of MSCs The cultured cells were differentiated into osteogenic, adipogenic and chondrogenic lineage by culturing in osteogenic medium [DMEM supplemented with 10− 8 M dexamethasone (Sigma, D4902), 10 mM β glycerophosphate (Sigma, G9422), and 50 μg/ml ascorbic acid], adipogenic medium [DMEM supplemented with 10 mM 3 isobutyl-1-methylxanthine (Sigma, 17018), 0.1 mM indomethacin (Sigma, 17378), 10 μg/ml insulin (Sigma, I6634), 10− 6 dexamethasone] and chondrogenic medium (Stempro, Invitrogen) and con rmed by staining with Alizarin red (Sigma, A5533), Oil red O (Sigma, O0625) and Alcian blue (Himedia Laboratories, Mumbai, India: Cat no RM471-1 g staining, respectively). .. Flow cytometry analysis For surface marker immunophenotyping, cells were stained with the following conjugated antibodies: anti-CD45-vioblue, anti-CD34-PE, HLADr-vioblue, anti-CD73-PE, anti-CD90-FITC, anti-CD105-vioblue, CD24PE CD44-FITC, CD133-APC, CD14-PE and relevant isotypes (Miltenyi-Biotec). ..

    Article Title: KRAS-driven pancreatic ductal adenocarcinoma cell lines harbour putative cancer stem cells
    Article Snippet: .. Single cells (1 × 106/100 μL) were stainedwith human CD44FITC (Cat# 130-113-334, RRID: AB_2726110, Miltenyi Biotec), CD24-PE (Cat# 130-095-953, RRID: AB_10828818, Miltenyi Biotec), and CD133-APC (Cat# 130-113-106, RRID: AB_2725935, Miltenyi Biotec) antibodies (Miltenyi Biotec, Germany) by diluting the antibodies at 1:50 ratio in fluorescence-activated cell sorting (FACS) staining buffer: 0.5 % (w/v) BSA (Sigma-Aldrich, USA), 2 mM EDTA (SigmaAldrich, USA) in 1 × phosphate-buffered saline (PBS; pH 7.4) at 4 °C in the dark for 10 min. Dead cells were stained with 5 μL (0.25 μg) 7-Aminoactinomycin D (7-AAD; BD Biosciences, USA) at 4 °C in the dark for 10 min. Data were acquired by using the BD FACSCantoTM II flow cytometer (BD Biosciences, USA) and analysed with BD FACSDivaTM software (version 8.0, RRID: SCR_001456, BD Biosciences, USA). ..

    Article Title: Autologous hGMSC-Derived iPS: A New Proposal for Tissue Regeneration.
    Article Snippet: .. Briefly, 3 × 105 cells/sample were stained for the following surface antigens: CD29–phycoerythrin (PE), CD31–fluorescein isothiocyanate (FITC), CD34 PE–Cyanin7 (CY7), CD45 allophycocyanin H7 (APC-H7), CD73 PE, CD90 FITC, CD106 FITC, CD117 PE, CD144 FITC, CD146 PE, CD326 peridinin-chlorophyll-protein (PerCP)–Cy5.5, HLA-ABC FITC, HLA-DR PE (all from BD Biosciences, San Jose, CA, USA); CD44 FITC, CD105 FITC, CD166 FITC (Ancell Corporation, Stillwater, MN, USA), and CD133 APC (Miltenyi Biotec, Köln, Germany), for 30 min at 4 ◦C in the dark. .. The staining of intracellular antigens was carried out on at least 3 × 105 cells/sample using the BD Cytofix/Cytoperm (cat. 554722.

    Fluorescence:

    Article Title: KRAS-driven pancreatic ductal adenocarcinoma cell lines harbour putative cancer stem cells
    Article Snippet: .. Single cells (1 × 106/100 μL) were stainedwith human CD44FITC (Cat# 130-113-334, RRID: AB_2726110, Miltenyi Biotec), CD24-PE (Cat# 130-095-953, RRID: AB_10828818, Miltenyi Biotec), and CD133-APC (Cat# 130-113-106, RRID: AB_2725935, Miltenyi Biotec) antibodies (Miltenyi Biotec, Germany) by diluting the antibodies at 1:50 ratio in fluorescence-activated cell sorting (FACS) staining buffer: 0.5 % (w/v) BSA (Sigma-Aldrich, USA), 2 mM EDTA (SigmaAldrich, USA) in 1 × phosphate-buffered saline (PBS; pH 7.4) at 4 °C in the dark for 10 min. Dead cells were stained with 5 μL (0.25 μg) 7-Aminoactinomycin D (7-AAD; BD Biosciences, USA) at 4 °C in the dark for 10 min. Data were acquired by using the BD FACSCantoTM II flow cytometer (BD Biosciences, USA) and analysed with BD FACSDivaTM software (version 8.0, RRID: SCR_001456, BD Biosciences, USA). ..

    FACS:

    Article Title: KRAS-driven pancreatic ductal adenocarcinoma cell lines harbour putative cancer stem cells
    Article Snippet: .. Single cells (1 × 106/100 μL) were stainedwith human CD44FITC (Cat# 130-113-334, RRID: AB_2726110, Miltenyi Biotec), CD24-PE (Cat# 130-095-953, RRID: AB_10828818, Miltenyi Biotec), and CD133-APC (Cat# 130-113-106, RRID: AB_2725935, Miltenyi Biotec) antibodies (Miltenyi Biotec, Germany) by diluting the antibodies at 1:50 ratio in fluorescence-activated cell sorting (FACS) staining buffer: 0.5 % (w/v) BSA (Sigma-Aldrich, USA), 2 mM EDTA (SigmaAldrich, USA) in 1 × phosphate-buffered saline (PBS; pH 7.4) at 4 °C in the dark for 10 min. Dead cells were stained with 5 μL (0.25 μg) 7-Aminoactinomycin D (7-AAD; BD Biosciences, USA) at 4 °C in the dark for 10 min. Data were acquired by using the BD FACSCantoTM II flow cytometer (BD Biosciences, USA) and analysed with BD FACSDivaTM software (version 8.0, RRID: SCR_001456, BD Biosciences, USA). ..

    Saline:

    Article Title: KRAS-driven pancreatic ductal adenocarcinoma cell lines harbour putative cancer stem cells
    Article Snippet: .. Single cells (1 × 106/100 μL) were stainedwith human CD44FITC (Cat# 130-113-334, RRID: AB_2726110, Miltenyi Biotec), CD24-PE (Cat# 130-095-953, RRID: AB_10828818, Miltenyi Biotec), and CD133-APC (Cat# 130-113-106, RRID: AB_2725935, Miltenyi Biotec) antibodies (Miltenyi Biotec, Germany) by diluting the antibodies at 1:50 ratio in fluorescence-activated cell sorting (FACS) staining buffer: 0.5 % (w/v) BSA (Sigma-Aldrich, USA), 2 mM EDTA (SigmaAldrich, USA) in 1 × phosphate-buffered saline (PBS; pH 7.4) at 4 °C in the dark for 10 min. Dead cells were stained with 5 μL (0.25 μg) 7-Aminoactinomycin D (7-AAD; BD Biosciences, USA) at 4 °C in the dark for 10 min. Data were acquired by using the BD FACSCantoTM II flow cytometer (BD Biosciences, USA) and analysed with BD FACSDivaTM software (version 8.0, RRID: SCR_001456, BD Biosciences, USA). ..

    Software:

    Article Title: KRAS-driven pancreatic ductal adenocarcinoma cell lines harbour putative cancer stem cells
    Article Snippet: .. Single cells (1 × 106/100 μL) were stainedwith human CD44FITC (Cat# 130-113-334, RRID: AB_2726110, Miltenyi Biotec), CD24-PE (Cat# 130-095-953, RRID: AB_10828818, Miltenyi Biotec), and CD133-APC (Cat# 130-113-106, RRID: AB_2725935, Miltenyi Biotec) antibodies (Miltenyi Biotec, Germany) by diluting the antibodies at 1:50 ratio in fluorescence-activated cell sorting (FACS) staining buffer: 0.5 % (w/v) BSA (Sigma-Aldrich, USA), 2 mM EDTA (SigmaAldrich, USA) in 1 × phosphate-buffered saline (PBS; pH 7.4) at 4 °C in the dark for 10 min. Dead cells were stained with 5 μL (0.25 μg) 7-Aminoactinomycin D (7-AAD; BD Biosciences, USA) at 4 °C in the dark for 10 min. Data were acquired by using the BD FACSCantoTM II flow cytometer (BD Biosciences, USA) and analysed with BD FACSDivaTM software (version 8.0, RRID: SCR_001456, BD Biosciences, USA). ..



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